STEAM Honors Design and Development Senior Capstone Project
PROJECT TRAILER
FILES
PROJECT PLAN
EXPERIMENT
To ensure that this product was effective, I did an experiment with the help of Mr. Weber, a biology teacher who has a degree in microbiology. The goal of this experiment was to determine how long the gloves needed to be exposed to the UV-C light to be thoroughly cleaned. Together we prepared petri dishes pouring the agar out and sterilizing the environment so that it would allow bacteria to grow. Then I rubbed some gloves against raw chicken for 30 seconds each and put them under the UV-C light for different periods of time. The first pair of gloves was not exposed to UV-C light after that we increased the exposure by 40 seconds each time. We had two trials with 0, 40, 1:20, 2, and 2:40. After I swabbed them, I gently brushed the swab on the petri dish. We incubated the petri dishes at about 90 degrees to allow the bacteria to grow.
We were surprised by how effective the light was at eliminating the bacteria, without warping the gloves. The ones that weren't exposed to the UV-C light for as long appeared to have salmonella and white E.coli clusters. E.coli can be distinguished by the crusty texture and salmonella tends to have a yellowish color. There was one outlier at 2 minutes which appeared to have a lot of bacteria, but this may have been an error on my part. However at 2:40 there was no bacteria present which shows how effective UV-C light can be in a short period of time.